Optimizing the condition of the PCR detecting of PTEN gene in DNA Research Paper - 1

Optimizing the condition of the PCR detecting of PTEN gene in DNA derived from human electric cell lines, associated with ageing and senescence - Research Paper ExampleTraditional PCR is used for amplifying DNA for sequencing, genotyping and cloning. However, this has a be of shortcomings namely poor precision, low sensitivity, short, dynamic range of less than 2 logs, low resolution, is non-automated, based on discriminatory size only, results cannot be expressed as numbers, the staining agent ethdium bromide is not cost-efficient at quantification and finally, post-PCR figure outing is required.This paper looks at the analysis of PTEN using traditional PCR method. The process was not boffo despite the necessary steps being undertaken. PTEN primer, when used on a Hacat cell line, showed annealing at approximately 62 degrees. However, the use of PTEN with similar parameters that were later altered for optimization yielded no results. The methodology has been discussed, and the g el results showing synthesized images have been included in this report. The discussion that covers the PCR process and its effectiveness has also been included. The fidelity of the PCR process has been discussed to extrapolate on the possible reasons for the failure of this analysis.Phosphatase and Tensin Homolog gene commonly referred to as PTEN gene provides the necessary instructions for the synthesis of a protein that is present in almost all body tissues (Sulis, 2003). The protein is significant in its affair as a tumour suppressor and aids in the regulation of cell division by inhibiting cells from diving and growing too fast or uncontrollably. PTEN is a phosphotase which modifies other fats and proteins through the removal of inorganic phosphate groups comprising of one phosphorus atom and three oxygen atoms (Cohen 1990).There are a number of medical checkup conditions that are linked to PTEN gene mutation including Cowden Syndrome and Bannayan-Riley-Ruvalcaba syndrome (Weary et al, 1972 Cohen 1990) which are often termed together as the PTEN hematoma tumor syndrome. The gene mutations that are responsible for these conditions are found in body